Direct shoot organogenesis from leaf explants of Populus deltoides and changes in selected enzymatic activities.
Identifieur interne : 000478 ( Main/Exploration ); précédent : 000477; suivant : 000479Direct shoot organogenesis from leaf explants of Populus deltoides and changes in selected enzymatic activities.
Auteurs : Saloni Sharma [Inde] ; M S Reddy [Inde] ; Anil Kumar [Inde]Source :
- Physiology and molecular biology of plants : an international journal of functional plant biology [ 0971-5894 ] ; 2020.
Abstract
The direct shoot organogenesis was achieved from leaf explant of two commercially important clones of Populus deltoides on MS medium enriched with 15 mg/l adenine sulphate, 5 mg/l Ascorbic acid, 250 mg/l (NH4)2SO4 (referred to as PD1 medium) supplemented with 2.5 µM each of 6-benzylaminopurine and indole-3-acetic acid. Higher shoot organogenic potential was recorded from the explants of clone 'G48' as compared to clone 'L34'. The age of leaf explant also affected the shoot organogenic potential, and maximum shoot organogenesis was recorded in case of 5th leaf from the top of microshoot. Histological studies revealed altered cell division resulting in the formation of meristematic pockets after 5 days of culture, these meristematic pockets grew into dome protuberances by 10th day. Organized shoots were visible after 15 days of culture. A clear three phases of shoot organogenesis viz induction (0-4 days), initiation and organization (4-10 days) and growth (11-16 days onwards) were observed. Marked variation in the activity of enzymes such as catalase, peroxidase, polyphenol oxidase and acid phosphatase was observed during these phases. The activity of these enzymes was found to increase in cultures grown on the medium resulting in shoot organogenesis during shoot development (after 7 days of culture).
DOI: 10.1007/s12298-019-00755-4
PubMed: 32153326
PubMed Central: PMC7036373
Affiliations:
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Reddy</name><affiliation wicri:level="1"><nlm:affiliation>TIFAC-Centre of Relevance and Excellence (CORE) in Agro and Industrial Biotechnology, Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala, 147004 India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>TIFAC-Centre of Relevance and Excellence (CORE) in Agro and Industrial Biotechnology, Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala</wicri:regionArea><wicri:noRegion>Patiala</wicri:noRegion></affiliation></author><author><name sortKey="Kumar, Anil" sort="Kumar, Anil" uniqKey="Kumar A" first="Anil" last="Kumar">Anil Kumar</name><affiliation wicri:level="1"><nlm:affiliation>TIFAC-Centre of Relevance and Excellence (CORE) in Agro and Industrial Biotechnology, Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala, 147004 India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>TIFAC-Centre 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selected enzymatic activities.</title><author><name sortKey="Sharma, Saloni" sort="Sharma, Saloni" uniqKey="Sharma S" first="Saloni" last="Sharma">Saloni Sharma</name><affiliation wicri:level="1"><nlm:affiliation>TIFAC-Centre of Relevance and Excellence (CORE) in Agro and Industrial Biotechnology, Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala, 147004 India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>TIFAC-Centre of Relevance and Excellence (CORE) in Agro and Industrial Biotechnology, Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala</wicri:regionArea><wicri:noRegion>Patiala</wicri:noRegion></affiliation></author><author><name sortKey="Reddy, M S" sort="Reddy, M S" uniqKey="Reddy M" first="M S" last="Reddy">M S Reddy</name><affiliation wicri:level="1"><nlm:affiliation>TIFAC-Centre of Relevance and Excellence (CORE) in Agro and Industrial Biotechnology, Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala, 147004 India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>TIFAC-Centre of Relevance and Excellence (CORE) in Agro and Industrial Biotechnology, Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala</wicri:regionArea><wicri:noRegion>Patiala</wicri:noRegion></affiliation></author><author><name sortKey="Kumar, Anil" sort="Kumar, Anil" uniqKey="Kumar A" first="Anil" last="Kumar">Anil Kumar</name><affiliation wicri:level="1"><nlm:affiliation>TIFAC-Centre of Relevance and Excellence (CORE) in Agro and Industrial Biotechnology, Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala, 147004 India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>TIFAC-Centre of Relevance and Excellence (CORE) in Agro and Industrial Biotechnology, Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala</wicri:regionArea><wicri:noRegion>Patiala</wicri:noRegion></affiliation></author></analytic><series><title level="j">Physiology and molecular biology of plants : an international journal of functional plant biology</title><idno type="ISSN">0971-5894</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The direct shoot organogenesis was achieved from leaf explant of two commercially important clones of <i>Populus deltoides</i> on MS medium enriched with 15 mg/l adenine sulphate, 5 mg/l Ascorbic acid, 250 mg/l (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> (referred to as PD1 medium) supplemented with 2.5 µM each of 6-benzylaminopurine and indole-3-acetic acid. Higher shoot organogenic potential was recorded from the explants of clone 'G48' as compared to clone 'L34'. The age of leaf explant also affected the shoot organogenic potential, and maximum shoot organogenesis was recorded in case of 5th leaf from the top of microshoot. Histological studies revealed altered cell division resulting in the formation of meristematic pockets after 5 days of culture, these meristematic pockets grew into dome protuberances by 10th day. Organized shoots were visible after 15 days of culture. A clear three phases of shoot organogenesis viz induction (0-4 days), initiation and organization (4-10 days) and growth (11-16 days onwards) were observed. Marked variation in the activity of enzymes such as catalase, peroxidase, polyphenol oxidase and acid phosphatase was observed during these phases. The activity of these enzymes was found to increase in cultures grown on the medium resulting in shoot organogenesis during shoot development (after 7 days of culture).</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32153326</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0971-5894</ISSN><JournalIssue CitedMedium="Print"><Volume>26</Volume><Issue>2</Issue><PubDate><Year>2020</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Physiology and molecular biology of plants : an international journal of functional plant biology</Title><ISOAbbreviation>Physiol Mol Biol Plants</ISOAbbreviation></Journal><ArticleTitle>Direct shoot organogenesis from leaf explants of <i>Populus deltoides</i> and changes in selected enzymatic activities.</ArticleTitle><Pagination><MedlinePgn>399-407</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s12298-019-00755-4</ELocationID><Abstract><AbstractText>The direct shoot organogenesis was achieved from leaf explant of two commercially important clones of <i>Populus deltoides</i> on MS medium enriched with 15 mg/l adenine sulphate, 5 mg/l Ascorbic acid, 250 mg/l (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> (referred to as PD1 medium) supplemented with 2.5 µM each of 6-benzylaminopurine and indole-3-acetic acid. Higher shoot organogenic potential was recorded from the explants of clone 'G48' as compared to clone 'L34'. The age of leaf explant also affected the shoot organogenic potential, and maximum shoot organogenesis was recorded in case of 5th leaf from the top of microshoot. Histological studies revealed altered cell division resulting in the formation of meristematic pockets after 5 days of culture, these meristematic pockets grew into dome protuberances by 10th day. Organized shoots were visible after 15 days of culture. A clear three phases of shoot organogenesis viz induction (0-4 days), initiation and organization (4-10 days) and growth (11-16 days onwards) were observed. Marked variation in the activity of enzymes such as catalase, peroxidase, polyphenol oxidase and acid phosphatase was observed during these phases. 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